DNA实验-免疫细胞表面抗原分子CD家族对照表(CD1-CD24(11页).doc
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1、-DNA实验-免疫细胞表面抗原分子CD家族对照表(CD1-CD24-第 11 页免疫细胞表面抗原分子CD家族对照表(CD1-CD247)Exalpha Biologicals produces and sells products for Life Science Research, including monoclonal and polyclonal antibodies, for use in immunohistochemistry, western blot, ELISA and Flow Cytometry. We offer over 800 products and, thro
2、ugh collaborations with other scientists as well as our own in-house R&D programs, Exalpha continues to bring leading edge products to market. Our family of CD antibodies combines quality and performance with reasonable prices to make our CD antibodies the best value in the market today. Our family
3、of Proliferation products offer easy all-in-one kits or stand alone antibodies to quantitate proliferating cells. Take a look at our website or call us for additional information.Note:no information is available on CDw145, CD181, CD182, CD185-194, CD196-199, CD211, CD214-216, CD218-220,CD237Backgrou
4、ndCD:cluster designation of monoclonal antibodies (clusters of differentiation)Designated at the 1st to 7th Workshops on International Human Leukocyte Differentiation AntigensThe last conference was in 2000. The next conference is in 2004.HLDA Workshops are the primary mechanism to characterize leuk
5、ocyte surface antigenic molecules and epies; erythroid antigens are now includedFor 1st to 6th workshops, antibodies were submitted to the organizing laboratory, coded and sent to participating laboratories for testing againstvariouscelltypes. For the 7th workshop, a CD designation could be establis
6、hed for a molecule if its gene hasbeen cloned and at least one specific monoclonal antibody hadbeen studied in the WorkshopInterpretation should be based on cellular distribution of staining, proportion of positively stained cells, staining intensity and cutoff levels.CD1Family of non-polymorphic MH
7、C class I-like glycoproteinsAlso member of immunoglobulin superfamilyOn chromosome 1q22-23 (not MHC linked)Has 5 different subsets, all noncovalently associated with 12 kd beta 2 microglobulinFunction:restrict T cell responses to certain antigens; may mediate thymic T cell developmentPositive staini
8、ng (normal):cortical thymocytes (70%), activated T cells, Langerhans cells, interdigitating dendritic cellsPositive staining (disease):pre T ALL with cortical thymocyte phenotype; Langerhans cell histiocytosisNegative staining:mature peripheral T cellsCD1aPositive staining (normal):Dendritic cells i
9、n dermis/epidermis of benign inflammatory skin disordersPositive staining (disease):Langerhans cell histiocytosis (fairly specific), myeloid leukemias, some B cell malignancies; dendritic cells in most peripheral cutaneous T cell lymphomas,AJCP 2001;116:72Negative staining:normal B cells, most cutan
10、eous peripheral B cell lymphomas (? reflects replacement of reactive pattern containing dendritic cells with a neoplastic pattern of B cells)Micro images:Langerhans cell histiocytosisMicro images (AJSP subscribers):pulmonary Langerhans cell histiocytosisMicro images (Hum Path subscribers):Langerhans
11、 cell histiocytosisReferences:AJSP 2001;25:630CD1bPositive staining (disease):myeloid leukemias and some B cell malignanciesNegative staining:normal B cellsCD1cPositive staining (normal):subset of normal peripheral B cellsPositive staining (disease):myeloid leukemias and some B cell malignanciesNega
12、tive staining:normal B cellsCD1dPositive staining (normal):thymus (low levels), bowelCD1eCD2Aka E rosette receptor, LFA-2 (leukocyte function antigen)Function:binds CD58 / LFA-3 on antigen-presenting cells, and induces costimulatory signals in T cellsAlso regulates T and NK-mediated cytolysis, inhib
13、its apoptosis of activated peripheral T cells, mediates T cell cytokine production, regulates T cell anergyPositive staining (normal):thymocytes (95%), mature peripheral T cells (almost all), NK cells (80-90%), thymic B cells (50%)Micro images:extranodal NK/T cell lymphoma, nasal typeCD2RCD2 epies r
14、estricted to activated T cellsPositive staining:activated T cells, ? NK cellsCD3Aka OKT3Function:complex (5 chains) of integral membrane glycoproteins assembled as a complex; has long cylasmic tail with antigen recognition activation motif; complex is then down regulatedAlso subdivided into delta, e
15、psilon, gamma subtypesCylasmic expression at early T cell differentiation, then membranous expressionMost specific T cell antibodyPositive staining (normal):thymocytes, peripheral T cells, NK cells; also Purkinje cells of cerebellumPositive staining (disease):80% of T cell lymphomasNegative staining
16、:gamma delta T cell receptors, most B cell lymphomasMicro images:CD3 epsilon-testicular NK/T cell lymphoma (figure 3D)Micro images (AJSP subscribers):achalasia, post-transplant lymphoproliferative disease in liverReferences:AJSP 2001;25:1413CD4Aka OKT4, T helper/inducerOn chromosome #12pNonpolymorph
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- DNA 实验 免疫 细胞 表面抗原 分子 CD 家族 对照 CD1 CD24 11
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