南京农业大学农业微生物学试题库及答案.pdf
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1、南京农业大学微生物学试题 注:1.1.答题前,请准确、清楚地填各项,涂改及模糊不清者、试卷作废。答题前,请准确、清楚地填各项,涂改及模糊不清者、试卷作废。2.2.试卷若有雷同以零分计。试卷若有雷同以零分计。一、名词解释(65):1.Colony 菌落2.Virus 病毒3.Biogeochemical cycle4.Medium 培养基5.Tansduction 转导6.Mycorhiza 菌根二、问答题(70 分)1.What are the major differences between gram-positive and Gram-negative bacteria?(5 分)什么是
2、主要差异在革兰氏阳性之间,革兰阴性菌?2.Distinguish between(a)phototrophs and chemotrophs,(b)Lithotrophs and organotrophs.(4 分)区分(一个)光能营养型,化能营养,(b 淋巴细胞)无机营养生物,有机营养菌。3.Yeast can grow both under aerobic and anaerobic conditions.What is the difference in energyproduction?(8 分)酵母能在需氧的,厌氧条件下增长。有什么不同在能源生产?4.Draw a typical b
3、acteria growth curve.Explain each phase.(8 分)In what circumstance,filtrationand pasteurization methods are useful?(6 分)画一个典型细菌生长曲线。说明每个相。(8 分)在什么环境,过滤,巴氏消毒法方法有用?5.To determine the viable counts of a soil sample,which method is the best?List theprocedures.(10 分)要确定一个土壤样品的可行的计数,哪种方法是最好的吗?列出的程序。6.The d
4、onor bacterial cell in a transformation is probably dead.Explain why.(2 分)7.What are the roles of a cloning vector,restriction enzymes,and DNA ligase in molecularcloning?(6 分)在分子克隆的克隆载体,限制性内切酶和DNA 连接酶的作用是什么?8.What does the term“antagonism”means?Explain the usefulness in biological control.(4分)9.Expl
5、ain the following terms in Rhizobium-legume symbiosis:infection,host specificity,effectiveness,competitiveness.How to evaluate the symbiotic effectiveness in green-house?(10分)在豆科根瘤菌共生的感染,宿主特异性,效益,竞争力。温室共生效益如何评价?10.List the taxonomic hierarchy of classification.(7 分)列出分类的分类层次。微生物学试题 本试题一共 2 道大题,共 2 页
6、,满分 100 分。考试时间 120 分钟。总分阅卷人核分人题号题分得分一二三四五六七八九十注:1.1.答题前,请准确、清楚地填各项,涂改及模糊不清者、试卷作废。答题前,请准确、清楚地填各项,涂改及模糊不清者、试卷作废。2.2.试卷若有雷同以零分计。试卷若有雷同以零分计。一、名词解释(65):1.Bacterial chemotaxis细菌的趋化作用2.Lichen 地衣3.Chemoorganotroph 化能有机营养生物4.Colony 菌落5.Biogeochemical cycle 生物地球化学循环6.Conjugation 接合二、问答题(70 分)1.What are the un
7、ique features of microorganisms gram-positive?(10 分)2.Why does Gram-staining procedure stain some bacteria purple-violet and others red?(10 分)3.Explain the term“medium”,what is the purpose to make a selective medium?(10 分)4.To determine the viable counts of a soil sample,which method is the best?Lis
8、t the procedures.(8 分)5.What are the roles of a cloning vector,restriction enzyme and DNA Ligase in molecular cloning?(12 分)6.Explain the following terms in Rhizobium-legume symbiosis:infection,host specificity,effectiveness,competitiveness.How to evaluate the symbiotic effectiveness in green-house?
9、(20分)复习题复习题Questions-1Questions-11What are the unique features of microorganism?2List several applied areas of microbiology.Describe the importance of microorganisms in eachof these applied fields.3Discuss the differences between prokaryotic and eukaryotic cells.4What are some major differences betw
10、een gram-positive and Gram-negative bacteria.5Draw a typical bacterial cell and identify all parts.6Define the following termsArchaea 古菌,flagellum 鞭毛,bacterial chemotaxis 细菌的趋化作用,capsule 荚膜,spore 孢子,cyst mesosome plasmid 质粒,simple staining简单染色,Gram staining 革兰氏染色,colony 菌落,lytic bacteriaphage 噬菌体裂解,
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